줄기세포 지방이식 Things To Know Before You Buy
줄기세포 지방이식 Things To Know Before You Buy
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Even though we have not tested PneumaCult™ media for use with murine epithelial cells, you will find publications that report this application.
Greatest effects are attained when limiting prolonged cell exposure to ambient temperature circumstances. Look at keeping unused cells inside a humidified incubator with 5% CO2 at 37°C when undertaking greater experiments.
Contact us to debate personalized assay remedies for many different other cell types to fit your certain needs.
Perform rapidly at this step to pool wells into a 15 mL tube. Note: For a large-scale dissociation, use a multichannel pipette to pool cells into a sterile reagent reservoir. Include the pooled wells into a fifteen mL tube.
Cross-portion histology followed by hematoxylin and eosin (H&E) staining to evaluate the thickness of the tiny or substantial airway epithelium
There are 2 Reside-tradition morphology indicators for good differentiation and readiness for additional probable characterization. These are definitely:
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With regards to the number of mucus accumulation, a next wash might also be needed. See how a mucus wash is carried out On this ALI tradition differentiation video clip (skip to 02:24) >
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To evaluate 가슴수술 the regional specificity of your smaller vs massive airway, you'll be able to carry out the subsequent assays:
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